ABASTRACT. The objective of this study was to evaluate the plasma lipid profile and plasma fatty acids of dairy cows receiving diets supplemented with annatto. A total of 32 Holstein cows (550 kg), distributed in a completely randomized design, were allocated to individual stalls and submitted to following treatments: C0 = no annatto; C4 = inclusion of annatto at 4 g kg-1 dry matter (DM) of diet (0.07 g bixin kg-1 diet); C5 = inclusion of annatto at 5 g kg-1 DM of diet (0.09 g bixin kg-1 diet); and C7 = inclusion of annatto at 7 g kg-1 DM of diet (0.12 g bixin kg-1 diet). Blood samples were collected via epigastric vein puncture, centrifuged, and frozen for subsequent analysis. The results indicate that the inclusion (p > 0.05) of annatto does not decrease the total cholesterol or low and high density lipoproteins. However, it impacts the profile of fatty acids, evidenced by the reduction (p < 0.05) in levels of hypercholesterolemic fatty acids viz, myristic acid and palmitic acid. It also causes an increase in the levels of arachidonic acid, rumenic acid, linoleic acid, and total polyunsaturated fatty acids. Therefore, bixin included in the diets of dairy cows induces changes in the plasma fatty acid profile.

ABSTRACT Promising species for aquaculture need to have their nutritional requirements defined. Considering that protein is the most expensive macronutrient in aquaculture diets, the present study aimed to estimate the protein requirement of curimatãpacu fed with diets containing two lipid levels. Thus, eight experimental diets were formulated to contain 20, 25, 30 and 35% of crude protein (CP) associated to 11 and 15% of crude lipids (CL) were formulated, constituting a completely randomized factorial design (4 CP x 2 CL) with three replicates per treatment. The juveniles (3.6 ± 0.01 g) were fed daily with 6% of the biomass, three times a day (08:00, 12:00 and 16:00 h) for 70 days. The protein requirement was estimated by the combination of the broken line and quadratic regression models. The CP concentration in the diets was the only factor influencing (P < 0.05) the growth and feeding efficiency variables of the fish, without interaction (P > 0.05) with the CL level of the diets. The requirement for weight gain and fish feed efficiency was estimated at 32.5 and 34.5% CP. Protein retention decreased (P < 0.05) in proportion to CP elevation. Fish fed 15% CL had higher (P < 0.05) body lipid deposition. On the other hand, the increase of CP of the diets decreased (P < 0.05) lipid deposition in juveniles. Therefore, it is recommended that young curimatãpacu between 3 and 27 g in weight should be fed diets containing 32.5-34.5% CP and 11% of CL, equivalent to a protein: energy ratio of 22-23 mg kJ−1

The objective of this study was to evaluate morphometric measures, carcass yields, absolute weights and yields of commercial cuts of carcass of kids with no defined breed. Eighteen castrated male goats, with average weight of 15 kg were used. The experimental period lasted 105 days. The experimental design was completely randomized; animals were allocated to three treatments: free grazing without supplementation; restricted grazing without supplementation and free grazing with supplementation. Animals were supplemented (1% of body weight) with forage cactus (Opuntia ficus - indica, Mill) and soybean meal (Glycine max). The feeding consisted of continuous grazing on caatinga. Supplemented animals had higher live slaughter weight and body score, and consequently higher morphometric measures in vivo and in carcasses. The supplementation provided carcasses with higher body weight and body score. For yields of carcass, there was no difference between treatments restricted grazing and grazing with supplementation; supplementation provided carcasses with higher yields. Supplemented animals had higher absolute weights for commercial cuts. Supplementation of grazing goats raised in the caatinga provides animals with higher body weight at slaughter, greater morphometric measures in vivo and in carcass and better body conformation. Therefore, for supplementation, the level used in this experiment is a good alternative in the creation of small ruminants during the dry period in the semi-arid region.

O objetivo neste experimento foi avaliar os efeitos da substituição do feno de coastcross (Cynodon sp.) por casca de soja sobre a digestibilidade aparente dos nutrientes, os parâmetros ruminais e o metabolismo de nitrogênio. Dezesseis borregos (PV = 40,0±5 kg) foram individualmente alojados em gaiolas metálicas para ensaio de metabolismo em delineamento experimental do tipo blocos completos casualizados. O experimento teve duração de 14 dias, sendo 10 dias para adaptação dos animais às rações experimentais e 4 para coleta das amostras. Os animais foram alimentados com dietas compostas de 50% de volumoso e 50% de concentrado com mesmo teor de fibra em detergente neutro (FDN). A casca de soja substituiu o feno de coastcross em 0, 33, 67 ou 100% da matéria seca. Os teores de casca de soja na dieta tiveram efeito quadrático sobre os consumos de matéria seca e FDN. Contudo, observou-se aumento da digestibilidade da MS e FDN, enquanto a concentração de ácidos graxos de cadeia curta, acetato e propionato não se alteraram e o pH ruminal e a concentração de N-amoniacal diminuíram. A substituição parcial do feno de coastcross por casca de soja aumenta o consumo de matéria seca e diminui o pH ruminal sem prejudicar a digestibilidade da MS e FDN.

The objective of this experiment was to determine the effects of replacing coastcross hay (Cynodon sp.) by soybean hulls on nutrient apparent digestibility, ruminal measures and nitrogen metabolism. Sixteen ram lambs (40±5 kg of body weight) were housed individually in metabolism crates and assigned to a randomized completely block design. The experimental period consisted of 14 days; the first 10 days were used to adapt lambs to treatments and 4 other days for data collection. Animals were fed diets containing 50% concentrate and 50% roughage, both with the same amount of neutral detergent fiber (NDF). Soybean hulls replaced coastcross hay by 0, 33, 67 or 100% in the dry matter. Dry matter (DM) intake and NDF showed quadratic effect when soybean hulls were added to the diet. However, DM and NDF digestibility increased, while short-chain fatty acids, acetate and propionate concentration did not change and ruminal pH and ammonia decreased. Partially replacing coastcross hay by soybean hulls increases DM intake and lowers ruminal pH without affecting DM or NDF digestibility.

Objetivou-se avaliar a composição químico-bromatológica e a digestibilidade aparente de dez híbridos de milho (DK265bm3, DK265, HS5, HS6, HTV2, HTV27, Anjou285, Mexxal, Pistache e Buxxil) cultivados no INRA (Unité de Génétique et d'Amélioration des Plantes Fourragères, Lusignan-France), em parcelas de 150 m², com três repetições. Para o estudo de digestibilidade in vivo, os ovinos foram alimentados com silagem da planta inteira dos híbridos de milho com três repetições. Os híbridos de milho foram avaliados antes de ensilados pelo método NIRS, em que se pode constatar que houve diferença (p < 0,05) entre os híbridos para a composição química. Quanto aos valores de digestibilidade in vivo, observou-se que, o DK265bm3 se destacou dos demais híbridos quanto aos valores de MS, MO, celulose, PC e da DIVMS.

The objective of this experiment was to evaluate the chemical-bromatological composition and apparent digestibility of ten hybrids of corn (DK265bm3, DK265, HS5, HS6, HTV2, HTV27, Anjou285, Mexxal, Pistachio and Buxxil) planted at INRA (Unité of Génétique Amélioration des Plantes Fourragères, Lusignan-France), in 150 m² areas with three replications. The digestibility study was conducted using sheep fed corn hybrid whole plant silage with three replications. Corn hybrids were evaluated before ensilage using NIRS, and a significant difference (p < 0.05) was observed among treatments for chemical composition. DK265bm3 showed higher values than other hybrids for digestibility of DM, OM, cellulose, NDF and for IVDMD.

O objetivo deste trabalho foi avaliar a influência de fontes protéicas sobre desempenho, características da carcaça e da carne de cordeiros confinados, e estimar a digestibilidade do amido de rações com alta proporção de grãos. Foram distribuídos 28 cordeiros Santa Inês, em blocos completos ao acaso, de acordo com o peso vivo e a idade, no início do experimento. As fontes protéicas foram os farelos de: soja, amendoim, canola e algodão, em dietas isonitrogenadas com 90% de concentrado e 10% de volumoso (feno de coast-cross). Na determinação da digestibilidade, foram utilizados quatro borregos em delineamento experimental em quadrado latino 4x4, e a digestibilidade do amido foi estimada a partir do teor de amido fecal. Não houve diferenças (p>0,05) quanto ao consumo de matéria seca, ganho de peso vivo médio, conversão alimentar, características da carcaça e da carne, entre as fontes protéicas avaliadas. A digestibilidade do amido apresentou coeficiente de determinação de 93%. Independentemente da fonte protéica utilizada, o teor de amido nas fezes é um indicador eficiente na estimativa da digestibilidade do amido de dietas com alta proporção de concentrado para cordeiros.

The objective of this work was to evaluate the influence of protein sources on feedlot lambs performance, carcass and meat characteristics, and to estimate starch digestibility of high grain diets. Twenty eight Santa Inês ram lambs were selected in a complete randomized block design, according to body weight and age in the beginning of the experiment. Protein sources were the following meals: soybean, peanut, canola, and cottonseed in isonitrogenous diets with 90% concentrate and 10% roughage (coast-cross hay). Four ram lambs (48 kg) were used to determine starch digestibility in a 4x4 latin square design, and starch digestibility was estimated by fecal concentration of starch. There were no differences (p>0.05) for dry matter intake, average daily gain, feed conversion, carcass characteristics and meat quality, among the protein sources evaluated. Determination coefficient for starch digestibility was 93%. Fecal starch content is an accurate indicator of the starch digestibility estimation in lambs feeding high grain diets, independently of the protein source used.